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The LG2 SDR comprises as many as 22 SNP markers and 13 SSR markers, covering a map distance of up to 40 cM and accounting for 58% of the total skewed markers in the map.
However, studies with gene markers covering a larger number of tumours are important.
We used 13 MS markers covering a region ~1 Mb and ~0.8 Mb centromeric and telomeric, respectively, to D6S265.
We designed and genotyped 140 microsatellite markers covering a region of ~14 Mb (between markers D5S666 and D5S436, Figure 1) in parents and probands pools.
In the present study, this map has been completed to end up with 467 markers covering a total distance of 1331 cM.
Sal05 contained a large conserved block of AtC4 with 12 markers covering a genetic length of 40 cM (51.4% of the total map length), and one conserved block of AtC1.
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Linkage studies are either performed as full genome screens with a dense set of genetic markers covering all chromosomes, or locally (fine-mapping) at a certain chromosomal area of interest.
Using the information on diverse genomic regions between BMMI806 and BMMI866, 138 informative SNP markers covering all chromosomes (except Y) in an average distance of 24.8 Mb were selected for genotyping the parents, F2 and the G3-animals.
The modest LD in this study, rarely extending beyond 5 kb, confirms that genome-wide allelic association studies will require a very dense set of markers covering all genes.
A total of 137 microsatellite markers covering all autosomal arms, excluding acrocentric arms, were analysed.
A genome-scan using 189 polymorphic microsatellite markers covering all 26 autosomes was conducted on 172 backcross ewes.
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