We selected specific markers considered to be associated with a poor outcome (see web extra on bmj.com).
In total, we identified approximately 80 features as markers considered to provide biological validation of the data set and analysis (Additional file 2).
The proportion of phenotypic variance explained by markers, considered to be a measure of heritability, indicates that these traits are at least partially heritable; however, we did not assess the accuracies of genomic predictions and the small animal sample size may have influenced these estimates of heritability.
A total of 793 SSR markers from the 799 primer pairs tested, the two sex-linked SCAR markers and eight indel/SNP markers were considered to be of sufficient quality to use for map construction.
After evaluation of the marker orders and their respective positions on the chromosomes, the average distances between common markers were considered, to place the other markers.
On each chromosome, 1,000 marker loci were randomly located and each segment between two markers was considered to harbor a potential QTL, giving 5,000 markers and 4,995 potential QTL in total.
These markers are considered to be tissue specific so a definitive set of markers that work robustly in all tissues is still lacking.
Tantalum markers were considered to be unstable if they moved more than 0.3 mm relative to the other tantalum markers between examinations.
In preparation for subsequent phospho-profiling experiments, we stained splenocytes from MRL and lpr mice at varying stages of disease (5 20 weeks) with antibodies specific to surface markers considered relevant to disease progression: B220, CD11b, Ly6C, Ly6G, Gr-1, CD19, IgM, CD21, CD3, TCRβ, TCRγδ, CD4, and CD8 [31], [31].
Under Isis's harsh interpretation of Islam, statues and grave markers are considered to be idolatrous.
Hence, these markers are considered to be ideal for conservation genetics, genetic diversity assessment, variety protection, and construction of high-resolution genetic maps to link phenotypic and genotypic variation.
