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None of the markers characteristic for undifferentiated hES cells were detected in the hES-MP cells.
However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COL1) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC.
We describe a set of differentiation markers characteristic for the AIY interneuron class and show that the ceh-10 paired-type and ttx-3 LIM-type homeobox genes function to regulate all known subtype-specific features of the AIY interneurons.
For example, MSCs derived from amniotic fluid express many cell surface markers characteristic for BM-derived MSCs including CD73, CD90, CD105, and major histocompatibility complex (MHC) class I [44].
However, these epithelial cells still express cell surface markers characteristic for thymic epithelial cells – i.e. EpCAM1.
Notably, FGF4 exhibited no posteriorizing effect on gut endoderm as determined by markers characteristic for anterior and posterior gut endoderm (data not shown).
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Relative liver signal intensity - liver-to-muscle SI ratio lower than 0.645 is a marker characteristic for the advanced iron overload with sensitivity of 89% and accuracy of 80% (fig. 11).
Flow cytometry immunoprofiling demonstrated that all preparations lacked expression of the haematopoetic, macrophage, and endothelial markers: CD14, CD19, CD31, CD34, CD45, and HLA-DR, but remained immunopositive for markers characteristic of MSCs (CD73, CD90, and CD105).
To establish the subcellular localization of CII after uptake, macrophages were subjected to subcellular fractionation by Percoll density gradient centrifugation, and subcellular fractions were analyzed for markers characteristic of different subcellular compartments.
In vitro, Sox2 activated promoter-luciferase reporter constructs for differentiation markers characteristic of Clara, ciliated, and goblet cells, Scgb1a1, FoxJ1, and Agr2, respectively.
Microsatellite marker characteristics for islands (Macrotus) and groups (Erophylla) are shown in Table 1 and pairwise Fst are provided with Euclidean distances between genetic populations in Table 2.
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