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Although greater success rates might have been achieved after PCR optimization for individual primer sets and a smaller proportion of microsatellite markers (e.g., [ 18]), our protocol reduced both time and resources spent identifying suitable markers by utilizing a single protocol.
In this study, we isolated a series of SNP markers by utilizing shotgun library approach (Staden 1979) and CATS method (Aitken et al. 2004; Li et al. 2009), both of which have been considered as efficient mechanisms for SNP discovery at the whole-genome level.
Considering the importance of foxtail millet, functional significance of TEs and the necessity of TE-based markers in genotyping applications, this study was performed to identify the different classes of TEs and develop molecular markers by utilizing the sequence information of TEs.
Linkage analysis between the development of macroscopically evident arthritis and the homozygosity of chromosome-specific microsatellite markers by utilizing the N2 generation of back-crossing the F1 generation of SKG and Mus musculus castaneus to SKG mapped the skg locus to the centromeric portion of chromosome 1, with the lod score of the locus as infinite.
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Furthermore, locus specific sequences can be readily obtained from the PLUG markers, and by utilizing PLUG markers as probes, it will be possible to quickly identify target clones from the huge BAC library for wheat.
To obtain a variant-based representation of the data, we repeated the network generation on marker scale by utilizing the set of variants associated with more than one distinct trait.
Although the superiority of ME over SE in CV2 can be explained by borrowing information from the same lines across environments (Burgueño et al. 2012), gains in CV1 may likely attribute to more accurate estimates of environment-specific marker effects by utilizing genetic correlation.
We demonstrated two approaches for rapid development of diagnostic markers for MAS by utilizing genome sequencing and re-sequencing data: (1) by genotyping and selecting markers from genetic linkage maps closely linked to genes of breeder interest, and (2) by marker mining from scaffolds bearing non-diagnostic markers.
For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes.
We further examined gene signatures with a prognostic CRC marker potential and by utilizing an integrated analysis approaches, we have identified eight gene candidates, including RPN2, HMGB1, AARS, IGFBP3, STAT1, HYOU1, NQO1 and PEA15.
Therefore we reasoned the high density of markers following enrichment on chromosome 22 should be addressed by utilizing markers limited to an adjacent spacing of >50 Kb.
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