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Inhibitory effects demonstrated are on: platelet function by aggregometry, exposure of activation and apoptosis markers by flow cytometry, biochemical pathway by malondealdehyde (MDA) estimation and protein phosphorylation by Western blot.
MP quantitation and phenotyping were carried out using cell-specific markers by flow cytometry using antibodies recognizing cell-specific surface markers.
In addition, we examined the Meg-Erythroid progenitor (MEP) in WT and Chk1+/− mice using established markers by flow cytometry, and found a ∼2-fold increase of these progenitors (Figure 4B).
To test this, DCs were co-cultured with either MCF-7 cells or hMSC or alternatively treated with IL-10 for 24 h followed by examination of maturation markers by flow cytometry.
To determine whether hypoxia-stressed gCSCs would alter the expression of MHC and co-stimulatory molecules on monocytes, which give rise to macrophages, we compared monocytes that were exposed to gCSC-conditioned medium under hypoxia and normoxic conditions and then analyzed surface markers by flow cytometry.
Their proper activation by ultrapure E. coli LPS (see Materials and Methods) under our experimental conditions was verified by monitoring their morphological change by microscopic observation (not shown), the induction of the CD40 and CD80 cell surface markers by flow cytometry [1] (Figure 1B) and the induction of the proinflammatory cytokines TNF-α, IL-6 and IL-12 by ELISA (Figure 1C).
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The EGFP acts as a sorting marker by flow cytometry in eukaryotic cells.
AF-MSCs, DAF-MSCs and AL cells were examined for the expression of SSEA-4 embryonic stem-cell marker by flow cytometry at different time points.
Representative dotplots of acquisition for each marker by flow cytometry, and the mean and standard deviation are shown on Figs. 2 and 3, for stem cells from the synovial fluid and membrane, respectively.
Quantification of vimentin (a mesenchymal cell marker) by flow cytometry showed that vimentin was expressed in most linneg cells of saline-treated lungs and bleomycin-injured lungs of day 12 (data not shown), suggesting that linneg cells did not contain epithelial cells but consisted primarily of fibroblasts and myofibroblasts.
Among the 13 different NK cell markers studied by flow cytometry analysis, the expression of CD244 and NKG2D increased significantly in the CD56bright NK population.
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