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1) BLASTing [39] the flanking sequence against the D. melanogaster genome sequence to identify which Muller element and thereby to what chromosome in D. mojavensis it belonged; 2) linkage mapping to allozyme markers assigned to chromosome by Zouros [40], [41]; 3) looking for close linkage between assigned markers and unassigned makers.
Different markers assigned to the same base position were removed.
Additional file 4: Molecular markers assigned to clones via PCR amplification or microarray hybridization.
Markers assigned to a linkage group were initially ordered using the reduced data set.
We first selected 42 BAC clones that contained markers assigned to 33 RH.
Distorted markers assigned to a LG were randomly distributed (Additional file 4).
Similar(24)
We note that in some instances the marker assigned to a drug coincides with what expected given the known drug target (Table 1, Markers and Target columns).
Starting from the existing order of a linkage group, each marker assigned to that linkage group by two-point analysis, but not present on the multi-point map, was tested in every possible location.
Using allelic homology with the 3,177 maternally derived markers, the paternal markers were assigned to chromosomal linkage groups, where possible.
For BTA29, out of the 253 markers mapped, 215 markers were assigned to BTA29 by the bovine genome sequence assembly.
Similarly for ICPH 2438, 26 of the 42 markers were assigned to 12 marker groups.
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