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As they put out into a glassy sea, familiar topographical markers are set aside.
On the left side of Figure 2E, the filter conditions 1 to 6 in Table S3B (colon-, ovand-, and pancreas-specific markers) are set to "inclusion = No" and the color patterns of the tags are almost white (low fold).
If these two markers are set aside we find that all genes expressed in odd-numbered rhombomeres are extinguished while those in even-numbered rhombomeres are unaffected by the kto mutation.
If the coefficients of the markers are set to zero or a low value in the training phase, they are excluded from the model and their genotype information is not required during the breeding phase.
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Allele calls for inconsistent markers were set to 0 in the offending nuclear families involved in the inconsistencies.
A list of useful markers is set out in Supplementary Table 2.
Markers were set according to cells stained with fluorochrome-conjugated isotype control antibodies (all from BD Biosciences).
Statistical markers were set using irrelevant isotype-matched controls as reference.
The scanning interval between markers was set at 0.5 cM, and the window size was set at 10 cM.
When all image markers were set, the program added the individual slice volumes, and total volume was recorded.
Limits for the quadrant markers were set based on negative populations and controls isotype (data not shown).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com