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Moreover, such experimental approaches to developing SSR markers are laborious, time-consuming and costly.
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Although each of these molecular markers supported the delineation of multiple species in this complex, with concordant cryptic species defined by all markers [ 14, 15], the application of these markers is laborious.
Further, previously developed conventional markers are anonymous, laborious to genotype (e.g. AFLPs and RFLPs), less reproducible (e.g. random amplification of polymorphic DNA, RAPD), require more time for development (e.g. genomic SSRs), and, more importantly, are less transferable between species.
However, these measures are laborious, time-consuming and costly, so alternative markers of glycaemia are routinely used in managing patients with diabetes, and these markers can be considered for use in subjects without diabetes.
Sequence Tagged Site content-based methods are laborious and require an extremely high density of markers.
Phone calls are laborious.
Prospecting and sales are laborious exercises.
These are laborious and time-intensive procedures.
However, such experimental methods are laborious, time-consuming and expensive.
These phenotypic markers are identified through laborious genetic screens.
Development of AFLP-derived STS markers tend to be laborious and time-consuming due to the lower conversion efficiency.
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