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Given that markers are isolated from coding regions, conservation is expected to be high, such that these EST SSR markers are generally also transferable to related species (e.g. Gupta et al. 2003).
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Cells expressing key β-cell markers were isolated by fluorescence-activated cell sorting after staining for high zinc content using the vital dye, Newport Green.
For β-glucuronidase activity analysis QC markers were crossed to rlk902 and plants homozygous for the rlk902 mutations well as for transgene markers were isolated from the F2 population and analyzed in the next generation.
Eight microsatellite markers were isolated using next-generation sequencing.
The BES reads from which the 126 mapped markers were isolated represent 88 unique BAC FPC contigs.
A total of 80 unique DNA fragments spanning AFLP markers were isolated and sequenced.
Methods and Results: Seventeen microsatellite markers were isolated by a microsatellite-enriched library protocol.
Microsatellite markers were isolated by following a high-throughput genomic sequencing approach developed by Abdelkrim et al. (2009).
Methods and Results: Eleven polymorphic microsatellite markers were isolated from an enriched library of V. reitzii and characterized.
Fourteen microsatellite markers were isolated and characterized from A. aurantiaca genomic libraries enriched for di-, tri-, and tetranucleotide repeat motifs.
Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides.
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