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The other markers are generated (again depending on the image and encoding parameters) by the Init_JPEG.
Microsatellite markers are generated from simple sequence repeats (SSRs) within the eukaryotic genome and are utilized via molecular genotyping for scoring genetic information (1).
We also expected some marker redundancy (markers with the same sequence) among the 3808 composite map DArT markers; an issue which arises due to the process by which DArT markers are generated, resulting in the same amplified genomic fragment being represented more than once on the genotyping array [ 31, 35].
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In general, 40 independent microsatellite markers were generated according to the Single Step Model; simulated population sizes were in the range between 100 and 10 000 and mutation rates were adjusted between 0.01 and 0.001 to reach the considered θ values.
A total number of 458 polymorphic segregating markers was generated with AFLPs.
Besides the SSRs, 208 AFLP markers were generated with 184 progeny.
To establish the genetic map for Family 331, a total of 848 markers were generated.
Variations of the cassette with different markers were generated (Table 1) to allow different combinations of fluorophores and selection markers.
T. brucei cell lines tagged with two drug-resistance markers were generated to select parasites that had stochastically activated an individually tagged BES from the background of highly similar BESs.
AFLP markers were generated after [ 80].
First, maps with sex-specific markers were generated based on 80 meioses and 1582 polymorphic markers.
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