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The custom fiducial markers are designed to be automatically localized in both patient and image spaces.
The Ownership Hypothesis for mammalian scent marking [25] predicts that some markers are designed to claim ownership or exclusive use of focal resources or sites.
Most of the mentioned studies are based on anonymous markers or markers falling outside genic regions, whereas our markers are designed within expressed genomic regions.
Cleaved amplified polymorphic sequences (CAPS) markers are designed based on restriction fragment length polymorphisms of PCR amplified fragments, when sequence information of one of the haplotypes is unknown [ 6].
Simple sequence length polymorphism (SSLP) markers, similar to SSR markers, are designed based on a unique segment of genomic DNA sequence that contains a simple tandem repeat that distinguishes the genotypes.
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All InDel markers were designed by Primer 5.0 software.
A multiplex PCR amplifying 11 polymorphic markers was designed.
The two markers were designed based on the insertion/deletion between Nipponbare and Kasalath.
InDel markers were designed based on the references genome of Nipponbare and 9311 reference sequences.
The sequences of the markers were designed using the PREMIRE5.0 software.
Nuclear INDEL markers were designed to distinguish O. meridionalis from Nipponbare (Additional file 2: Figure S2).
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