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To further understand the relevant proteins/intermediate filaments expressed in ferret neocortex, we tested several other markers and observed that BLPB was strongly expressed.
Correlations between polymorphic markers and observed phenotypes provide the basis for mapping traits in quantitative genetics.
KININFOR v.1.0 [ 152] simulated the power of applied markers and observed allele frequencies to discriminate between unrelated and full-/half-sib individuals.
A total of 322 contigs were mapped with multiple markers and observed recombination between at least one pair of markers, allowing contig-based estimations of ratios of physical to genetic distance (Table S2).
To investigate the characiform radiation, Ortí and Meyer [ 19] used partial mitochondrial rDNA sequences (870 bp) as phylogenetic markers and observed that two citharinoids (Distichodus and Citharinus) were more closely related to Siluriformes than to characoids.
We have also performed the colocalisation staining between synovial 4-HNE and all cellular specific markers and observed 4-HNE expression in T-cells, B-cells, macrophages and cells of blood vessels.
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We quantified the number of EGFP transfected lymphocytes using the CD3 marker and observed 1.0±0.2% of cells in lymph nodes and 1.5±0.2% in the spleen (Figure 3F).
We also looked for gene expression of catalase, an oxidative stress marker, and observed that in Hemin-treated group there was a diminished amplification of this molecule, when compared to UUO group (Figure S2, panel G).
Cells were immunostained with anti-V5 (red, FUS) and DAPI (blue, nuclear marker) and observed under confocal microscopy.
Next we used anti-PfHsp70-1 (Mattet et al., 1989) as a cytoplasmic marker and observed a colocalization with PfSUMO (Fig. 4A, middle panel).
In support of this, we immunostained mouse embryos with Lamin-B1, a nuclear envelope marker, and observed the formation of Lamin-B1 encapsulated micronuclei in Msk2 MO-injected, but not 3′COF MO-injected embryos prior to lysis (Fig. 5F).
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