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The markers and map developed will potentially be useful more broadly in the genus Tigriopus.
LG1 was obtained as 2 sub-groups, LG1a and LG1b, with 6 and 2 markers, and map lengths of 29 and 0.3 cM, respectively.
LG6 was obtained as 3 sub-groups, LG6a, LG6b and LG6c, with 4, 2 and 2 markers, and map lengths of 9, 3 and 2 cM, respectively.
Here, we backcross the region around ws1 g from N. giraulti into N. vitripennis using newly available visible markers, and map a major host preference effect in the region.
Numbers of markers and map lengths were significantly different among the three genomes, with 1152 markers covering 1698.7 cM in A-genome, 1265 markers covering 1687.1 cM in B-genome, and 237 markers covering 680.5 cM in D-genome.
Primer sequences for the microsatellite markers and map positions were obtained from the UniSTS database of the National Center for Biotechnology Information (Supplementary Table 4); primer sequences for the SNPs were designed using Primer3 software (v0.4.0).
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Materials | Computers with Internet access, projection equipment to view videos, poster board and markers, and maps of your city or county.
To further demonstrate the ease of the RAD sequencing approach we identified polymorphic markers and mapped an induced mutation in Neurospora crassa.
Combining whole-genome resequencing and genome-wide association study (GWAS) would help identify markers for quantitative trait loci (QTL); however, recombination mapping is still needed for validating previously identified markers, identifying new markers, and map-based cloning approaches.
Browse genetic markers and maps.
ZHT and WCY screened the SSR markers and mapping.
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