Postnatal skin specific inactivation of Notch1 resulted in loss of skin appendages, deregulation of several epidermal differentiation markers and hyperproliferation (Figure S1) as previously reported [5].
We were also able to confirm that normal human dermal fibroblasts and human fetal fibroblasts (MRC-5) also express CD68 (originally described as a monocyte/macrophage marker) and CD164 (Figures 1 and 7), as described previously [51] [54].
The identity of these cells is confirmed by double labeling for T cell and macrophage markers (see Figures 5 and 6 below).
Across all analyses, three associations were found between markers and heading date (Figure 2), two of which were detected by haplotype analyses only.
ESC differentiation was accompanied by gradual downregulation of pluripotent markers and expression of lineage markers (Supplementary Figures S1E and S2C).
The analysis showed a good predictive accuracy of those markers (Table 8 and Figure 6).
The map spanned 365.67 cM with an average interval of 7.78 cM between adjacent markers (Table 3 and Figure 1).
Each of these ER markers exhibits partial, but not perfect, overlap with other ER markers (see Figures 4E and 4F and Additional file 1) [ 10].
We confirmed that female EpiSCs had undergone XCI, and contained an Xi, while expressing pluripotency markers (Supplementary Figures S3 and S4B).
In particular, ECFP co-localized with keratin-8 and keratin-14, two prominent cortical and medullar markers, respectively (Figure 4A and 4B).
