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After 4 weeks, SDF-1 alpha treated tendon had increased expression of tendon repair gene markers and endogenous SDF-1 alpha, exhibited more physiological microstructures with larger diameter collagen fibrils, and had better biomechanical properties than the control group.
cDNA macroarrays containing probes for 83 genes (including undifferentiated hESC and hepatic markers and endogenous controls) were fabricated as described [29].
Genetic analyses by use of biochemical and immunological markers and endogenous murine leukemia virus (MuLV) proviral markers revealed that each SAM strain constitutes a genetically distinct group.
Lack of complete chloroplast genome sequences to provide 100% homologous species-specific chloroplast transformation vectors, containing suitable selectable markers and endogenous regulatory elements, is one of the major limitations to extend this concept to other useful crops [ 22, 23].
However, colorimetric separation of signals among colocalizing markers, and between markers and endogenous pigments, is almost impossible, and so, only double staining, or triple staining for limited cases [ 29], can be applied.
As choroid plexus epithelial cells contain a large number of mitochondria and show extensive Golgi complexes and endoplasmic reticulum, which appear to traverse from basal to apical membranes [ 70, 71], it is now proposed that HRP (and other markers and endogenous molecules) translocate through plexus cells via a transcellular pathway utilizing these networks [ 71].
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We used Spearman's rank correlation to evaluate associations of the xenoandrogenic activity both to POP markers and to endogenous serum estradiol and testosterone (total and free), which served as method verification that the sex hormones were excluded from the F1 fraction.
This gene set comprises 82 validation targets from Tag-seq analysis, eight glioma and developmental markers, and three endogenous control genes (18S ribosomal RNA, TUBB and NDUFB10).
The clones were tested for the expression of pluripotency markers by PCR and endogenous alkaline phosphatase activity (Vector Laboratories).
To date, various methods are available to measure tumour oxygenation, including polarographic oxygen sensors (Vaupel et al, 1991), luminescence-based optical sensors (Bussink et al, 2000) and antibody-based detection of exogenous (Haustermans et al, 2000; Raleigh et al, 2001; Koch, 2002) and endogenous markers of hypoxia (Wykoff et al, 2000; Janssen et al, 2002; Airley et al, 2003).
Consistently, our results showed that GF-iPS-3F cell clones highly expressed ES cell marker genes, including Nanog and endogenous Oct3/4.
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