Early detection of cancer is also a promising application of epigenetic markers, and comparison of sensitivity and specificity with markers now used in clinical practice is necessary.
An assessment of the efficacy of MRI screening is required using surrogate markers and comparison with comparable high risk groups as performed in the Dutch study.
The most widely used approaches rely on amplification of a defined panel of multi-allelic markers and comparison to similar data from other samples.
Although association mapping may provide valuable information on associated markers and comparison with known DArTs may suggest their chromosomal assignments, their precise location is difficult to determine if saturated consensus genetic maps are not available.
The locations of conserved genes on the other grass genomes enabled syntenic regions to be identified while mapping of known EST & SSR markers and comparison with syntenic regions from Brachypodium enabled the order of syntenic genes in the chromosome to be assessed.
This would allow further investigations in the light of new information after the studies ended; for example, if subsequently a new marker is discovered, then the tissue samples could be assessed for this marker and comparison made to the existing markers.
Interestingly, Ochrobactrum is the Brucellaceae member closest to Brucella according to several molecular markers and genome comparisons [14].
Upon sequencing 20 of these marker regions and comparisons with the H37Rv genome sequence, 25% appeared to share homology to members of the antigenically variable PE/PPE surface protein encoding gene family confirming previous findings on the genetic heterogeneity within these genes.
Our approach is horizontal in two senses: a comparison was made across diverse markers and a comparison was made across different forms of evidence.
Genotyping was performed with 38 markers and a comparison was made with 16 backcross lines and three RILs (Fig. 3).
