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Markers adapted for high-throughput genotyping are available.
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PCR-based markers adapted to large-scale genotyping systems can be designed for constructing genetic linkage maps.
A panel characterized with a marker density adapted for GWAS is now available for public use [seeds from PRC's co-author and GBS data from TropGeneDB (see section on " Availability of supporting data section" for access)] and constitute a highly valuable resource for mining new alleles within Vietnamese genetic resources.
Development of image analysis procedures: Image analysis procedures for quantifying the 11 immunohistochemical markers will be adapted for each marker from our automated image analysis procedures developed in previous studies (see point 7).
SNP markers are well adapted for high throughput methods for marker saturation.
SSR markers are multiallelic and well adapted for fine analysis of population diversity structure.
To establish a matrix adapted for GWAS, markers with a minor allele frequency (MAF) below 5% were discarded.
The proposed platform is envisaged to prove useful in developing novel and robust image bio-markers which could then be adapted for use on the mMR scanner.
These results suggest that cyclin B1 is a strong independent prognostic marker that could add to accuracy of prognostic evaluation made by traditional prognostic markers and that could easily be adapted for routine use.
We identify a large pool of SNPs among 26 WCR populations, and show that these SNPs can be readily adapted for use as genotyping markers.
Thus, the system can be readily adapted for imaging existing fluorescent marker proteins as well as endogenous fluorophores (autofluorescence) and fluorescent dyes used for staining various cellular components, both in vivo and after fixation of the specimens.
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