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For effects on ACh synaptic function, we assessed three markers: activity of choline acetyltransferase (ChAT), cell membrane binding of hemicholinium-3 (HC3) to the presynaptic high-affinity choline transporter, and the concentration of α4β2 nicotinic acetylcholine receptors (nAChRs).
Selection stringency can be improved by using mutant neomycin phosphotransferase II selection markers with reduced affinities for the neomycin drug [ 32, 33], by using a weak Herpes simplex virus thymidine kinase promoter [ 34], and by codon deoptimization of selection marker gene [ 35], which reduce the selection markers' activity, transcription initiation and translation rate respectively.
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The myocardial infarct area, oxidative stress markers, activities of myeloperoxidase (MPO) and creatine kinase (CK) were determined after the experiment along with the assessment of the effect on apoptotic activity by using DNA fragmentation analysis.
Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages.
The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl4-induced reproductive toxicity in rats.
Cell marker activity was compared between ES cells and mouse embryonic fibroblasts (MEFs).
Such unique expression patterns are likely attributable to the effect of the selection marker activity on the overall cell physiology.
Clearly, yield and enrichment calculations become less dependable when they are based on a tiny fraction of the marker activity.
With these modules, we first asked how the selection marker activity is affected by the relative arrangement and the activity of the gene expression cassette.
In contrast to that, adding 15 to 25 mg silver powder showed a marked decrease in marker activity compared to titanium alloy control [ 11].
Sucrase (EC 3.2.1.48, enterocyte apical membrane marker) and sodium-potasium-ATPase (EC 3.6.1.3, enterocyte basolateral membrane marker) activities were determined in BBMV suspension [ 2].
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