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Depending on the selected marker, we obtained sequences for up to 430 of the sampled individuals, including up to eight markers (a total of 2198 sequences).
After evaluating and manually curating all markers, a total of 55,201 of the markers produced successful assays.
To identify and develop BAC-anchored microsatellite markers, a total of 13,581 microsatellites were detected from 10,355 BES.
Based on six markers, a total of six different MLVA genotypes (A-F) could be discriminated (Table 3).
Of the 485 markers, a total of 464 could be mapped on the soybean reference genome (Glyma1.01) covering 880 Mb (92.6%) of the genome length.
The LDA described above was then performed for the raw IC50 values, previously suggested risk indicators, and the simulated markers, a total of 761 possible markers.
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To determine whether G250 antigen (MN/CA IX/G250) could be a potential therapeutic target and a tumour marker, a total of 147 cases of RCC were investigated immunohistochemically as well as by reverse transcriptase polymerase chain reaction (RT-PCR) analysis.
Finally, we analyzed a combined data set containing all the previous markers (translation-related proteins plus our new markers, for a total of 70 markers and 9,540 amino acid sites).
The marker analysis that was conducted utilizing 1,349 randomly chosen SSR markers revealed a total of 76 markers that were polymorphic between the Li 2 parental NILs.
The integrated haploid map had 5377 nonduplicated markers and 1266 duplicated markers with a total map length of 3602.6 cM (File S2).
Among the successful 197 markers used for the hybridization were 87 markers that have been genetically mapped; these markers anchored a total of 54 contigs that cover 76.4 Mb (25.5% of the genome) on all 7 linkage groups (Table 4).
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