Sentence examples for markers a permeabilization from inspiring English sources

For the detection of intracellular markers, a permeabilization step was performed prior to the staining procedure.

By pulsed electric field (PEF) application a permeabilization of plant, animal and microbial cells is achieved.

The blood was then treated with a permeabilization reagent (IntraPrep™ permeabilization reagent IM2388) and stained with DAP-12 Alexa-647 conjugated antibody.

Noteworthy, our protocol included a permeabilization step before adding the inducers.

To this aim, we performed immunofluorescence colocalization analysis between MAPK15 and endogenous autophagic vesicle markers, upon permeabilization of cells with digitonin in order to eliminate the background signal due to their cytoplasmatic forms.

The latter would have a permeabilization effect on lysosomal and mitochondrial membranes.

Subsequently the cells were washed in a permeabilization solution followed by PBS to remove the detergent.

Cells were then stained with extracellular markers, followed by permeabilization and incubation with anti-mouse IL-10 APC, IFNγ APC or IL-17 PE mAbs.

Multistep sequential labeling was performed to label and fix cell-surface markers followed by permeabilization for cytokeratins (CK) 8, 18 and 19.

Further, the regulatory capacity of the FOXP3+ putative Tregs could not be examined as staining for this marker requires permeabilization, and isolated Tregs would thus not be viable.

Subsequently, this stain also acted as a surrogate marker for DV permeabilization.