Similar results, concerning type-2a/type-2b progenitor cells, were also obtained using Tbr2 as marker, which labels quite selectively this population [60], (Figure S2A-D).
To characterize the immune response we investigated cellular phenotype and proliferation in PBMC from Cohorts 1 and 2. Proliferation was measured using Ki67, an intracellular marker which labels cells that traversed the S-phase of the cell cycle (past 2 3 days) in combination with various cell surface markers to identify T cells (CD3+) and B cells (CD3-, CD20+, HLA-DR+).
To do this, we used double live confocal imaging of the LzGAL4, UAS-GFP marker, which only labels crystal cells and the HmlΔDsRed.nls marker, which labels plasmatocytes but not mature crystal cells.
To establish the neuronal compartments in which the WASH and retromer complexes are present we used the MAP2 marker, which labels the cell body and dendrites but not the axons.
Using short-term lineage tracing, we first assessed if nkx6.1+ cells can give rise to the first wave of endocrine cells of the dorsal bud using the Isl1 marker, which labels all mature endocrine cells.
In order to better understand how the new junctions are formed during asymmetrical cytokinesis, we investigated the dynamics of the cell membrane on the apical and basolateral side, using the Tg BAC: kdrl:mKate2-CAAX)UBS16 mKate2-CAAX UBS16 al., 2013) together with the Tg(kdrl:H2B-GFP) (Kochhan et al., 2013) marker, which Lenardcetl membrales and chromatin, respectively.
The neurosphere assay also enabled us to test the sensitivity of the hypoxic marker pimonidazole hydrochloride (PH), which labeled groups of cells in the SGZ.
We have the glial marker ERG1, which labels all radial glia, but this is not the case with neuronal component of the the echinoderm CNS.
The late bone differentiation marker osteocalcin, which labels mature osteoblasts, is downregulated in the stump of amputated fins and then robustly re-expressed in the proximal differentiated regenerate [ 12].
