We identified 182 studies, which assessed a total of 165 candidate genomic markers.
There are a number of PET response markers currently available, e.g. [F]fluorothymidine, which assesses cellular proliferation, H2O for assessment of angiogenesis and I-labelled annexin V for apoptosis.
Reproducibility of the IHC scoring was assessed using 20 slides for each marker which were independently assessed by another pathologist (A. L). masked to J. P.'s results.
Microvessel density is one such marker, which can be assessed after staining specific to an endothelial cell-specific marker, such as CD31, CD34 or CD105.
By elucidating the value of serum GGT as independent prognostic parameter for the survival in patients with endometrial cancer, we provide an additional serum marker, which can be routinely assessed within pre-treatment medical examination.
These are the so-called 'dynamic' markers, which can be assessed using electronic devices (such as FloTrac/Vigileo).
Another important aspect for outbreak investigations and for long-term surveillance studies is the stability of the assessed markers, which is not the case for all markers in MLVA (Dimovski et al. 2014) but which was demonstrated for our MOL-PCR assay by the results of a serial passage experiment.
To fully evaluate these factors in concert with molecular and genetic markers (which for example, may assess inflammation and associated genes), larger studies in consortial settings will be required.
Other markers which were used to assess the quality and quantity of the preparations included AKT (cytosolic fraction) and GAPDH (loading control for total lysate).
For this purpose, 1826 individuals from 49 breeds were genotyped using 21 microsatellite markers, which allowed us to assess the genetic diversity of sheep breeds in France in relation to their history and conservation policy issues.
