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Another recent study suggested that PTEN IHC was a potential predictive marker when stained on metastatic but not on primary tissue as used in the present study[ 16].
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When stained for neuronal markers, the human fetal-derived primary cultures not only expressed MAP2 and NeuN but also markers for immature neurons, Tuj-1 and Tuc-4.
Healthy kidney: when stained with Ki-67 marker, in contrast to glomerules and interstitial cells, tubular cells showed no proliferation.
The Ptbp2-KO neurons grew extensive neurites during the first week in culture and were indistinguishable from control cultures when stained with the dendritic marker MAP2.
Tartrate-resistant acid phosphatase (TRAP) is a marker of osteoclasts and shows a red color when stained with tartrate and chromogenic substrate.
When staining for the lymphatic marker LYVE-1 [27], we observed a marked increase in the number of lymph vessels in the allografts (Figure 4G I) indicative of lymphangiogenesis.
When we stained for the senescence marker β-galactosidase we found that in control fibroblasts of comparable passage numbers less than 1% of the cells were positive.
Indeed, when we stained Tg KRT5-CreER); Rosa26R-fGFP Tg KRT5-CreERan NE marker, we identified lineage-traced NE cells.
In contrast, when multiple fluorescent markers are used for staining, stained specimens cannot be stored for a long time due to the poor stability of fluorescent dyes.
Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.
Influence of digestion with collagenase on mast-cell surface-marker staining.
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