As an example, gastric surface mucous cells (GSM06) develop a characteristic columnar cell shape with an apical mucous layer accompanied by further epithelial differentiation markers when cultured in ALI culture conditions (Ootani et al. 2000; Yokoyama et al. 2007).
Ballios et al. recently demonstrated that a subpopulation of cells derived from the CE and sorted out on the basis of size and pigmentation criteria could be induced to express high levels of immature and mature photoreceptor markers in a sequential manner when cultured under specific differentiation conditions for extended periods of time (up to 40 days).
Our recent study showed an upregulation of myogenic markers of myoblasts when cultured on the nanopatterned substratum and an increase in dystrophin expression when nanopatterned monolayered muscle patches were implanted in an in vivo mouse muscular dystrophy model, relative to unpatterned controls.
We first assessed whether the presence of SAP affected the loss of CD93 and gain of 25F9 expression on PBMC, two markers that change rapidly when cultured in vitro (Figure 4 and data not shown).
Similar observations have been reported for MC3T3-E1 pre-osteoblastic cells, which showed higher expression of markers of osteoblast differentiation when cultured on rigid TCPS (2 GPa) compared to 424 and 14 kPa hydrogels [16].
The main goals of this study were to ascertain whether the MSC from UC Wharton's jelly had the capacity to synthesize the specific markers of functional odontoblast when cultured in TGC-CM in vitro.
The expression levels of the primitive markers CD34 and CD133 remained similar when cultured in either serum-free or serum-containing media (Figure 2B), although a decrease in CD34 expression was seen for cells cultured in serum containing media after 7 days (72% in the presence of serum versus an average of 98% in serum-free media, data not shown).
In contrast, cells sorted for CD44+/CD24- or CD133+ markers lost their stem cell phenotype when cultured in monolayers.
When cultured, these cells expressed the pluripotency markers SOX2, OCT4 and SSEA-4 (figure 1A-A″').
In this paper, we first demonstrated that not all NSCLC cell cultures are able to form tight spheroids when cultured in spheroid medium, and that this ability correlates with the level of activity of the CSC-like marker ALDH1A1.
