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Morphological and functional evaluation and western blot analysis of EMT marker were performed upon peritoneum tissue.
Immunostains for each marker were performed on a Dako Autostainer (Dako Corporation).
Singleplex reactions for each marker were performed using identical thermocycling reactions as described above.
Quality controls at three different concentrations for each marker were performed daily.
Univariate analyses for each clinical variable and each molecular marker were performed using Kaplan Meier curves and log-rank tests.
Immunostains for each marker were performed in a single staining run on a Dako Autostainer (Dako Corporation, Carpinteria, CA, USA).
Similar(47)
For each sample, quantification of cells positive for a given marker was performed by analysis of 5 10 representative regions of the section.
In order to evaluate the accumulation of Lodamin in its micelle structure at the CNV lesion site, a comparison between free or encapsulated fluorescent marker was performed.
Initial genotyping and screening for loss of the selectable marker was performed by PCR using Expand HiFi (Roche) according to the manufacturer's recommendations.
Immunohistochemical staining with Ki67, a cell proliferation marker, was performed to analyze cell proliferation.
Plot of allelic frequencies for each location and marker was performed with the above package.
More suggestions(15)
marker were distributed
marker were located
marker were scaled
marker were propagated
marker were explored
marker were analyzed
marker were extracted
marker were dissolved
marker were measured
marker were maintained
marker were obtained
marker were found
marker were detected
marker was performed
marker were grown
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