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Exact(15)
Reagents used in polymerase chain reaction (PCR), including long and accurate LA-Taq DNA Polymerase, PCR-grade dNTPs, restriction enzymes, T4 DNA ligase, and DNA ladder marker, were obtained from Takara (Dalian, China).
Strains containing multiple disruptions with the same auxotrophic marker were obtained from genetic crosses and confirmed by PCR.
The +/gcs1 plants were crossed with the transgenic Arabidopsis expressing HTR10-RFP (sperm nucleus marker), and the F1 +/gcs1 plants hemizygously expressing the marker were obtained.
Cutoff points for each tumor marker were obtained with ROC analysis.
Sequence statistics for specified regions of each marker were obtained utilizing SeqState [ 75].
PCR product lengths for each marker were obtained by using Genemarker software.
Similar(45)
A marker-free hLF BAC vector containing the entire hLF genomic sequence without an antibiotic resistance marker was obtained in our previous study, which contains the procedural details17.
Finally, a full-length cDNA clone containing an engineered SacII site that served as a genetic marker was obtained.
Precision Plus Pre-stained molecular weight marker was obtained from Bio-Rad.
Dig-labeled RNA size marker was obtained from Roche.
Polymorphism level of 2.4 alleles per marker was obtained.
More suggestions(15)
indicator were obtained
benchmarks were obtained
marker were normalized
marker were scaled
marker were explored
marker were analyzed
marker were extracted
marker were dissolved
marker were considered
marker were measured
marker were assembled
marker were positioned
marker were maintained
marker were counted
marker were wounded
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