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Cells carrying the natMX4 marker were grown on YPD containing 75 µg/ml nourseothricin (Jena Bioscience, Germany).
WT and PS1-hp mutant strains containing either pDK243 LEU2 (1X-ARS site) or pDK368-7 (8X-ARS sites) with a LEU2 marker were grown in synthetic complete media leucine to stationary phase, then plated onto YEPD and synthetic complete media leucine to determine plasmid stability under selection.
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Campylobacter jejuni strains with antimicrobial resistance markers were grown on kanamycin (30 µg/ml) or chloramphenicol (4 µg/ml) when appropriate.
The use of molecular markers are growing as a diagnostic tool in brain tumors due to their potential to overcome limitations inherent to both pathological and clinical assessments [18].
Bloodstream-form T. b. brucei cells (strain 427, 'single marker') were grown at 37 °C and 5% CO2 in a modified HMI9 medium [10] (HMI9-T where 0.2 mM 2-mercaptoethanol was replaced with 0.056 mM thioglycerol).
As an additional precaution, populations of opposite Ara marker types were grown adjacent to one another.
When using the amdS as selection marker, strains were grown in MM without NaNO3 and supplemented with 10 mM acetamide and 15 mM cesium chloride.
When using the amdS gene as selection marker, strains were grown in MM in which the 70 mM NaNO3 was replaced with 10 mM acetamide and 15 mM cesium chloride (Meyer et al. 2010).
For analysis of epithelial-to-mesenchymal transition (EMT) markers, cells were grown under the nonpermissive conditions in complete medium for 24 hours, then switched to medium with reduced serum (0.5% FBS) for a further 18 to 24 hours and, finally, were treated with 2 ng/ml TGFβ for 48 hours prior to isolation of RNA and protein.
(B ) Cells expressing copper-inducible GFP-CFTR and the ER marker DsRed-HDEL were grown in copper-containing medium for 2 hr and then visualized.
Cultivars of L. angustifolius employed for genome re-sequencing and marker validation tests were grown from single-seed-descent derived self-pollinated lines to minimize heterogeneity.
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