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Eight small linkage groups together containing positive BAC IDs for each typed marker were generated at a very stringent threshold of LOD = 6.0 (Figure 3).
To establish a series of technology for production of stable cMAbs and to evaluate which type of expression vector gives us a maximum performance for cMAb production, four major different expression systems with a DHFR amplification marker were generated by re-engineering the pIRES-DHFR vector.
Alignments for each marker were generated with Muscle [ 95] using the default settings.
To determine whether the expression profile of the HAE::GUS abscission zone marker is also rescued in as1-20 bp-1 flowers, mutants carrying this marker were generated.
The CFP-GUS cytoplasmic marker and the CFP nucleocytoplasmic marker were generated following amplification of the CFP coding sequence using primers CFP-for and CFP-rev (Additional file 8).
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A fluorescent marker is generated in neurons in which genetic recombination was previously induced in the dividing precursor cells.
Each size marker was generated by digesting genomic DNA (10 µg) with restriction enzymes as indicated in figure legends.
A fluorescent marker was generated in neurons at all stages of the Drosophila life cycle using a highly efficient and controlled genetic recombination system that can be induced in dividing precursor cells (MARCM system, flybase web site).
The curve of the ideal marker was generated assuming arbitrarily as 1 its plasma level at the value of GFR measured in CKD patients at stage 1 (111.3 ml/minute/1.73 m).
hrd1Δ strain PEY1756 marked with natMX marker was generated by transforming linearized pFA6a-NatMX into PEY893 and mating with KGY461 to switch the mating type.
scp1Δ strain PEY1748 marked with natMX marker was generated by transforming linearized pFA6a-NatMX into PEY554 and selecting on medium containing 100 μg/l clonNAT.
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marker were normalized
marker were averaged
marker were analyzed
marker were explored
marker were extracted
marker were dissolved
marker were considered
marker were calculated
marker were positioned
marker were assembled
marker were counted
marker was generated
marker were obtained
marker were wounded
marker were detected
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