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In 1995, criteria for the diagnosis of AL with biphenotypic marker were established by the European Group for the Immunological Characterization of Leukemia (EGIL) explicitly [ 3], and then this scoring system was improved for AL with ambiguous lineages in 1998.
The C-terminal truncated twk-1 :: gfp and controls were directly injected into twk-1 (tn1397 )/ unc-13 (e51 ); acy-4 (ok1806 )/ nT1 [ qIs51 ] animals, and several transgenic lines expressing the str-1 :: gfp co-injection marker were established.
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The protocol for analysis of this marker was established at the research laboratory.
Phases of all the linkage group markers were established by the 'linkage phase establishment' function in the CarteBlanche software (Keygene, Wageningen, Netherlands).
GPS and picket markers were established at each sampling site, and forest block aerial images and rehabilitation timelines were provided by the company.
The levels of immunoreactivity for all markers were established as numerical variables.
Call rates for individual markers were established by the TGAS program for call rates over all cases and controls.
Based on the gametic recombination of each parent, two parental maps composed of SSR and DArT markers were established.
Once linkage groups of markers were established, predefined haplogroups of tightly linked loci (i.e. LOD ≥ 3.0 and theta ≤ 0.03) were identified using HAPLOGROUP.
Microsatellite markers were established for several species within the family (e.g., Barbará et al., 2007; Gürcan and Mehlenbacher, 2010), but not for C. betulus.
Two molecular markers were established using traditional marker development methods, which were linked to Lanr1 gene at the genetic distance of 3.5 and 2.3 centiMorgan (cM), respectively [ 12, 19].
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