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The Gn1a-17 SNP marker and GS5-03 SNP marker were developed based on this method.
In a second step SNP marker were developed for as many as possible of those genes and used to anchor them on the barley linkage map.
Primer pairs for each marker were developed with the PRIMER 3 software and tested for optimal amplification using a temperature gradient (from 58-67° 58-67°
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The marker was developed from a RAPD-PCR amplification product unique to isolate GV4.
In the molecular markers module, the search page of PGD molecular marker was developed based on available public data resources containing a large number of IP, SSR, and SNP markers.
The Pi9-Pro marker was developed based on InDel polymorphisms.
b The dCAPS marker is developed according to the smg11 mutation.
And, for selection of the GS5-WG allele, the GS5-03SNP-FD marker was developed.
The C21200d marker was developed to confirm the mutation in pgr7.
At least one SSR marker was developed from each BAC.
An SSR marker was developed to investigate cosegregation of alleles more distantly positioned relative to FaFAD1.
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