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Using the methods described by Karagiannis and Balasubramanian diploid S. pombe strains bearing one wild-type and one synthetically constructed mutant copy of the rpb1 gene (marked with the ura4+ selectable marker) were created (Table S1).
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When clicked, the marker will be centered the screen and the image will automatically zoom to the level at which the marker was created.
For this, DNA constructs with a selectable marker are created with sequences that are 5′ and 3′ to the kinase coding region for targeting specific recombination.
A phylogenetic marker was created by concatenation of individual alignments of single-copy families derived by mafft (Katoh et al. 2002) (L-INS-I algorithm).
As the bubbles were indistinguishable from each other under both OCT and CCD imaging, a negative fiducial marker was created when needed, by popping several bubbles on the edge.
For the VINO-only tree, synthetic markers were created at a small distance from each real marker (0.001 cM).
InDel markers were created based on polymorphisms between these two ecotypes to meet the demands of researchers.
The REMAP markers were created by combining LTR primers with ISSR primers, as shown in Table 3.
All PMA1 or pma1-105 sthatns that expressed other markers were created by backcrossing to these original haploids.
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CEO of Professional Science Editing for Scientists @ prosciediting.com