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Using this approach, segregation data for 729 marker were assembled on 43 LGs.
Deletion cassettes consisting of 1.0 1.5 kb fragments of gene-specific flanking regions interrupted by the selection marker were assembled by yeast recombinational cloning as described in detail by Schuster et al. [ 43].
The PCR product of the right arm, the DNA fragment containing the R-Luc-fused left arm and the PGK-Zeocin selectable marker were assembled and finally cloned into an AAV2 proviral plasmid, giving rise to a vector that harbors 2.0 kb of TNF-α genomic DNA fused in frame to the R-Luc cDNA and with a zeocin cassette inserted at the center.
Similar(57)
A deletion cassette containing 5' and 3' fragments of the CAT gene and the URA3 marker was assembled in pDEST43 with Gateway LR reaction using plasmids pENTR_41_5'CAT, pENTR_221_URA3 and pENTR_ 23_3'CAT.
Markers were assembled into sets of six loci suitable for multiplex PCR.
SSR and AFLP markers were assembled into a genetic linkage map with JOINMAP 3.0.
Markers were assembled into linkage groups at likelihood ratio statistic (LOD) ≥ 3.0 and assembled into a consensus map.
These GBS markers were assembled into 26 linkage groups, forming genetic maps for the winter wheat DH population (Table 1).
We analyzed whether positive BAC clones of single locus RFLP markers were assembled into the same segment of a contig.
Furthermore, to generate a unique set of EST markers in Triticeae plants among the public domain, 3472 markers were assembled to form 2737 unique marker groups as contigs.
Of these, 621 markers were assembled into 19 linkage groups (LGs) for the maternal map, 696 for the paternal map, and 1 254 for the integrated map.
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