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Given that Proxison exhibited some co-localization with a mitochondrial marker, we decided to test its protective capabilities directly against a known mitochondrial antioxidant, MitoQ35.
Considering the distal position of the marker, we decided not to examine rs267413 further in this study.
Because KRT16 was found to be strongly overexpressed in RDEB and aging and was known to be a pathology marker, we decided to study it also at the protein level by immune fluorescence methods.
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Among the five candidate genes discovered in the proximity of outlier markers, we decided to examine further the cadherin (CAD) and leucine aminopeptidase (LAP) at the sequence and expression levels, to confirm or infirm the selection footprint.
Since this binary distribution probably represents the real-world situation among strains and also favors the practical applicability of analyzing selected markers, we decided to continue our analyses by converting our linearly distributed data into binary values representing the presence or absence of each marker.
Because of significant overlaps of cell populations based on epithelial (E-cadherin/EpCAM) or mesenchymal markers (CD44/vimentin), we decided to use an indirect approach to isolate E/M cell subfractions.
In consideration of the two different aspects of GAL3 and of BIVA (the first is a fibrosis marker, and the second is a dynamic marker of congestion), we decided to evaluate in this study the degree of congestion correlated to that of fibrosis in AHF patients alone or together for the prediction of events.
As necrosis is an indirect marker of hypoxia, we decided to study the in vitro effect of experimental hypoxia on renal cancer stem-cells sorted from these renal cell carcinoma xenografts.
A commonly used biochemical marker of apoptotic cell death is the induction of caspase 3. Since caspase 3 is an "executioner" caspase and is a reliable marker for impending apoptosis, we decided to evaluate the levels of caspase 3 in HIGK cells infected by A. actinomycetemcomitans deficient in the CDT compared to the wild-type infection.
Moreover, since the characteristics of peripheral arteries, like thickness of intima-media layers of carotid wall, are used as surrogate markers for coronary atherosclerosis, we decided to compare directly gene expression of these two vessels.
Alix is a protein that is widely considered to be a valid marker of exosomes [9]; therefore, we decided to investigate its presence in the exosomes purified from the culture media.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com