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The diagnostic reliability of the new female specific marker was verified on 54 different genotypes.
The insertion of the antibiotic resistance marker was verified using PCR with two different primer sets, each containing a primer that binds within the antibiotic resistance gene and a second that binds either upstream or downstream of the intended insertion site.
Integration of the marker was verified by southern blot and genetic analyses.
The value of securin as a proliferation marker was verified immunohistochemically (n=44) in invasive ductal breast cancer.
Proper integration of the S. cerevisiae URA3 marker was verified using a reverse PCR primer that overlapped the URA3 marker (either URA3-CHECK or URA3-CHECK#2) and forward PCR primer that was complementary to genomic sequences upstream of the 5′ region used to perform homologous recombination (termed CgZCXX-check, see Table S1).
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The D-genome specificity of the markers was verified by positive amplification in hexaploid DNA (SW58; AABBDD) and no amplification in tetraploid wheat DNA (LDN; AABB).
After two successive plant-to-plant passages (21 days each), viral DNA was extracted from single plants as described below, and the integrity of all six genetic markers was verified by sequencing, confirming their stability.
PAI-1 also modulates insulin signaling in fibroblasts, preventing the binding of vitronectin to avb3 receptors that, in turn, reduces insulin-induced phosphorylation of protein kinase B. 4, 5 A positive correlation between plasma PAI-1 concentration and insulin resistance (IR) markers was verified by epidemiological studies.
Marker verification A group of markers are verified together for a control transfer instruction.
All four markers were verified for species specificity, and three (H1, H2B and MARS) presented a single and clear PCR amplicon for the two dreissenid species (see Supplementary Fig. S2).
Identified markers were verified with both RT PCR and ISH on a range of TGCT samples.
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