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To make mapping calculations more tractable, only one marker was retained from a set of markers at the same genetic position and individuals with more than 50% missing data were removed.
A study carried out several years previously, showed that the introduction of FGF2 to the growth media enhanced expression of the O4 antibody in cultured OECs and that the marker was retained for several weeks (Alexander et al., 2002, Fig. 4).
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Only one of a set of redundant markers was retained.
The correlation structure of the markers was retained between each permutation.
In addition to this core content, additional modules of SNPs were added sequentially so that maximal economy of markers was retained by ensuring no redundant SNPs were added.
As a result, only 9303 SNP markers were retained and used for linkage and QTL mapping.
A total of 9303 SNPs markers were retained and used in the linkage map construction (Fig. 2, Additional file 1: Table S1).
Here, we show that the expression of these markers is retained in adult cultures 5 days after FGF2 withdrawal, when CholAB is added at the time of FGF2 withdrawal (Figure 7a c).
After this first filtering step, 18,818 markers were retained.
At the end of the editing procedure, 30 055 markers were retained.
After this filtering, 17,372 high-quality SNP markers were retained (Dryad Digital Repository: doi 10.5061/dryad.461nc).
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