Stable integration of the SV40-Neo selection marker was proven by PCR amplification with primers for the SV40 promoter and the Neomycin resistance (Figure S1).
Several genes were reported hypermethylated in CRC, althought no gene marker was proven to be individually of sufficient sensitivity or specificity in routine clinical practice.
In the study, the triple multiplex system on coding region SNP markers was proven to be very useful for analysis on forensic materials [ 2].
However, this question will not be solved until the predictive value of these markers is proved in a series of patients with different evolutive status and secondly until the consistency of the results in different muscles and different laboratories is proved.
The subnetwork markers were proved to be more reproducible than individual marker genes and achieved higher accuracy in the classification.
The DArT markers were proved to be a powerful diagnostic tool to study the genotypic diversity in Foc.
In the end, the most promising markers were proved (1) to amplify, and (2) to be repeatable and polymorphic (i.e., bearing at least two alleles per population); the remaining were monomorphic, nonamplifying in some individuals, or too unspecific.
Two different kinds of molecular markers are proving very useful for these studies.
Most of these markers were proven polymorphic, some of them were localized in the polytene chromosomes of the species and many of them were successfully cross-amplified in other Tephritidae species.
It typically takes 10-15 years or more to grow trees to the point where they can be selected to develop improved or new varieties; therefore, if such markers are proven effective, they could potentially reduce the selection time and cost.
