Your English writing platform
Discover LudwigSuggestions(5)
Exact(38)
Counterselection of the URA3 marker was performed by cultivation on 5-fluoroorotic acid containing plates [5-FOA plates: 6.9 g/L YNB without amino acids (Formedium), 0.77 g/L complete supplement mixture (Formedium), 20 g/L glucose and 0.8 g/L 5-fluoroorotic acid (Sigma)].
For each sample, quantification of cells positive for a given marker was performed by analysis of 5 10 representative regions of the section.
In order to evaluate the accumulation of Lodamin in its micelle structure at the CNV lesion site, a comparison between free or encapsulated fluorescent marker was performed.
Initial genotyping and screening for loss of the selectable marker was performed by PCR using Expand HiFi (Roche) according to the manufacturer's recommendations.
Immunostaining for each marker was performed in 30 µm parasagittal spinal cord sections according to the stereological principles described above (one in six sections), resulting in an average of 4 sections, per marker, that contained a visible injury epicenter.
To assess MVA-B infection in MDDC, flow cytometry analysis of intracellular Gag protein and cell-surface CD86 as a MDDC marker was performed 16 h post-infection at a dose range of 0.003 3.3 or at a single dose of 10 PFU/MDDC (Figure 1B D).
Similar(22)
Morphological and functional evaluation and western blot analysis of EMT marker were performed upon peritoneum tissue.
Immunostains for each marker were performed on a Dako Autostainer (Dako Corporation).
Singleplex reactions for each marker were performed using identical thermocycling reactions as described above.
Quality controls at three different concentrations for each marker were performed daily.
Univariate analyses for each clinical variable and each molecular marker were performed using Kaplan Meier curves and log-rank tests.
More suggestions(15)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com