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The clpP:: cat marker was introduced to stabilise the RseP-cleaved fragment of HA-MBP-RseA148.
An erythromycin resistance marker was introduced into a pRB373 shuttle vector.
This would be the first time that the phospho-specific DNA-PKcs marker was introduced as an indicator for cell senescence.
In DY4792, a natMX marker was introduced by PCR-based gene targeting so that it replaced the genomic DNA between coordinates 2127216 and 2127259 on chromosome 2 in the intergenic region between SPBC23G7.14 and rpp202.
A targeting vector was designed in which loxP sites were introduced upstream and downstream from Pcdh15 exon 38, and a neomycin resistance (neo) cassette flanked with Frt sites as selectable marker was introduced downstream of exon 38.
Similar(55)
An extrachromosomal array consisting of this construct, Pglh-2:: Mos1 transposase, and three fluorescent mCherry negative selection markers was introduced into Mos-1 ttTi5605 Mos-1 ttTi5605ms via microinjection into the germline of young adults.
In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification.
For S. bayanus, auxotrophic markers were introduced by EMS mutagenesis as described previously (Zill and Rine 2008).
The markers are introduced and explained in the process of examining them through the high classes of the ICPS framework.
While the order could be manually changed, the size of the linkage group was often greatly increased and large breaks between markers were introduced.
When DNA-containing selectable markers are introduced into the cell, they integrate randomly throughout the genome, allowing the generation of mutants containing exogenous DNA insertions.
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