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While the MSNF9-GFP transgene was not activated in the hematopoietic organs of control larvae (Fig. 4A), the lamellocyte marker was induced in lymph glands obtained from wasp-infested animals (Fig. 4B).
Indeed, we found that p-Stat3, an involution marker, was induced in Ncadk.i.i
In our experiments, we demonstrated clearly that PARP cleavage, an apoptotic marker, was induced in cerulenin-treated U251 and SNB-19 cells; this was associated with significant cytotoxicity.
Again, the biochemical results showed that cleaved caspase 3, an apoptotic marker, was induced by Pink1 knockdown and reduced by Pink1 overexpression under thapsigargin conditions.
Figure 1D shows, as expected, that following retinoic acid treatment, expression of the pluripotency factor OCT4 was lost and NES, a neuroectoderm marker, was induced.
Notably, the collagen type II marker was induced in all cell samples isolated from BM whereas only five out of eight samples from the synovial tissue were positive after differentiation (data not shown).
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Low levels of circulating IL-10 is consistent with other reports that this anti-inflammatory marker is induced and not necessarily expressed under non-stimulated conditions [ 30, 31].
After 5 days, expression of differentiation markers was induced and quantified by quantitative PCR (qPCR) and flow cytometry.
Under these conditions, the expression of PGC markers was induced at about 50% of the level of that without inhibitor (Fig. 2G).
A block in the autophagic process, evidenced by the accumulation of LC3-I, LC3-II and p62, essential autophagy markers, was induced by PES.
In MCF-7 cells, phosphorylation of the anti-apoptotic gene and persistent upregulation of pro-apoptotic markers was induced by the Dox→Pacl sequence and further enhanced by exposure to 5-FU.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com