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Each marker was genotyped twice.
The marker was genotyped in the British study which showed similar effect size than the Finnish sample.
Each marker was genotyped twice and a third genotyping was performed in case of discrepancy between the first two experiments.
When a putative co-segregation was identified, the marker was genotyped over all the progeny that had been phenotyped for fruit colour.
Both parents in the Br5 family were informative; therefore, the microsatellite marker was genotyped in this family using an ABI 377 automated DNA sequencer.
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Alleles of this marker were genotyped using allele-specific probes and primers purchased from Applied Biosystems (Catalogue # C__26719686_10).
A large set of SNP markers was genotyped using the GoldenGate assay; collectively, this set is referred to as "gSNPs".
For this reason, a number of additional markers was genotyped in each mapping population to increase the number of common markers among them.
Brain tissue from 100 pools of individuals were arrayed with Affymetrix U74Av2 arrays chips and a panel of 779 markers was genotyped.
Highly polymorphic microsatellite markers were genotyped by semi-automated fluorescent genotyping techniques with the ABI3700 machine and Genotyper® software from Applied Biosystems as previously described [23].
To perform interval mapping for the families with significant contrasts, 21 additional markers were genotyped on chromosomes 2, 7, and 27.
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