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Each size marker was generated by digesting genomic DNA (10 µg) with restriction enzymes as indicated in figure legends.
A fluorescent marker was generated in neurons at all stages of the Drosophila life cycle using a highly efficient and controlled genetic recombination system that can be induced in dividing precursor cells (MARCM system, flybase web site).
hrd1Δ strain PEY1756 marked with natMX marker was generated by transforming linearized pFA6a-NatMX into PEY893 and mating with KGY461 to switch the mating type.
scp1Δ strain PEY1748 marked with natMX marker was generated by transforming linearized pFA6a-NatMX into PEY554 and selecting on medium containing 100 μg/l clonNAT.
The curve of the ideal marker was generated assuming arbitrarily as 1 its plasma level at the value of GFR measured in CKD patients at stage 1 (111.3 ml/minute/1.73 m).
The mitochondrial marker was generated by combining the first 29 amino acids of the yeast cytochrome c oxidase IV ScCOX4 and the coding sequence of the mEGFP (mEGFP-Cox) according to Kölher et al. [ 44] and Nelson et al. [ 30].
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A fluorescent marker is generated in neurons in which genetic recombination was previously induced in the dividing precursor cells.
Eight small linkage groups together containing positive BAC IDs for each typed marker were generated at a very stringent threshold of LOD = 6.0 (Figure 3).
Alignments for each marker were generated with Muscle [ 95] using the default settings.
To determine whether the expression profile of the HAE::GUS abscission zone marker is also rescued in as1-20 bp-1 flowers, mutants carrying this marker were generated.
The CFP-GUS cytoplasmic marker and the CFP nucleocytoplasmic marker were generated following amplification of the CFP coding sequence using primers CFP-for and CFP-rev (Additional file 8).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com