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After 7 days in culture, the ability of the cells to take up Ac-LDL, an endothelial marker, was evaluated by fluorescence microscopy (Fig. 6B).

The double-immunostained slides were scanned using the ScanScope® slide scanning system (Aperio Technologies Inc., Vista, CA) and images analyzed by a Color Deconvolution Algorithm (Aperio Technologies Inc .. Expression levels of each marker was evaluated by two pathologists (ADN and SS) blinded to clinical and laboratory data.

This marker was evaluated in 644 patients.

The efficacy of CRPv as a diagnostic marker was evaluated by using receiver operator curves (ROC).

Co-localization of GFP-LC3 dots and signals for LAMP1, a lysosomal marker, was evaluated in the liver after CLP.

The clinical significance of neuron-specific enolase (NSE) as a tumour marker was evaluated in 54 patients with seminoma.

Models of molecular evolution for each marker were evaluated in MrModelTest v2.3 [ 26].

The activation of p53 and apoptosis markers was evaluated by Western blotting.

The expression of keratocyte markers was evaluated by real-time PCR, immunofluorescent staining and western blotting.

Defense gene expression, often using the PR1 and PBZ1 genes as markers, was evaluated for a large number of cases (37/55).

Expression of cancer stem cell markers was evaluated by flow cytometry.