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The protocol for analysis of this marker was established at the research laboratory.
Since the evidence indicates that BrdU might affect the studied cell population, when its use as a birth-date marker was established, much attention was given to its dosage and the frequency of exposure, in order to minimize toxicity and avoid misleading results (e.g., [ 10, 11, 22– 22]).
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In 1995, criteria for the diagnosis of AL with biphenotypic marker were established by the European Group for the Immunological Characterization of Leukemia (EGIL) explicitly [ 3], and then this scoring system was improved for AL with ambiguous lineages in 1998.
The C-terminal truncated twk-1 :: gfp and controls were directly injected into twk-1 (tn1397 )/ unc-13 (e51 ); acy-4 (ok1806 )/ nT1 [ qIs51 ] animals, and several transgenic lines expressing the str-1 :: gfp co-injection marker were established.
The presence of specific markers was established to characterize the HPTEC primary cultures.
This positioning of markers was established by selection for mitotic crossing-over in heterozygous diploids.
The cutoff for all cell surface markers was established based on isotype controls.
Once an initial list of nine DIP STR markers was established (Table 1), we tested single-marker allelic variability by genotyping 20 Swiss individuals under informed consent.
In this report, a transient transformation system for the orchid lip using markers derived from different species as fluorescent markers was established to study subcellular localization of proteins.
Once the utility of these markers was established, the remaining individuals of T. dubius, T. miscellus, and T. pratensis were PCR-amplified and digested in the same manner.
A panel of 24 microsatellite markers was established [See Additional file 1: Table S2], according to the recommendations of FAO and the International Society for Animal Genetics ISAGG) [ 17].
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