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As an example, the SPIKE-01SNP marker was designed without the artificial mismatched nucleotide in allele-specific primers that were shown on the gel image.
A 300-bp B1SM-F/B1SM-R SCAR marker was designed based on the sequence alignment of the B17 products.
To identify the Wx-mq genotype simply and rapidly, a cleaved amplified polymorphic sequence (CAPS) marker was designed.
A novel dCAPS marker was designed in accordance with a base A deletion at site 316 of the sequence.
The pigeon mtDNA MST marker was designed to target the mtDNA Cytochrome b gene by employing mismatch amplification mutation assay kinetics.
Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males.
Similar(39)
Two overlapping and independent sets of primers that covered the whole sequence of each mitochondrial marker were designed, the corresponding fragments ranging between 70 and 115 base pairs.
A multiplex PCR amplifying 11 polymorphic markers was designed.
To perform an assessment of the method, a calibration model, with embedded fiducial markers, was designed in order to allow an accurate estimate of the 3D pose as a baseline.
A 3D microfluidic electrochemical origami immunodevice (denoted as µ-ECOI in this work) for sensitive detection of tumor markers was designed.
All InDel markers were designed by Primer 5.0 software.
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