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Once a marker was assigned to the cucumber draft genome scaffold, its map location was inferred in two ways.
Two of the markers, KS50140 and KR50161, anchored on this contig belonged to linkage group R3 but KS10551 marker was assigned to R9.
Secondly, if a marker had not been mapped in cucumber but the cucumber scaffold possessed markers that have been mapped in the cucumber genome, then that marker was assigned to the same location as those markers.
Each marker was assigned into linkage groups on the IMNpRH212,000-rad SSC10 map at a 2 pt LOD score of 8 and a threshold distance ≤ 100 cR between markers.
Similar(56)
In forensic DNA tests, each marker is assigned a number to designate its type.
As such, a hypothetical "cytoplasmic marker" was added to the dataset; alleles for this marker were assigned according to the maternal parent of each RIL.
For the interpretation of multiplex data, each marker that discriminates through presence or absence and each SNP marker is assigned a unique prime number.
We observed one instance of a marker being assigned to a different chromosome as reflected by its best BLAST hit to D. melanogaster.
The retrieved cDNA sequences corresponding to each EST marker were assigned to the rice genome to predict an exon-intron structure.
An initial panel of 530 SNPs with fixed genotypes in both parents were identified using these criteria and alleles for each marker were assigned to their respective parental donor (Additional file 2: Table S2).
The SSR markers were assigned to linkage groups (LGs) based on a maximum recombination fraction of 0.34 and a minimum logarithm of odds (LOD) score of 6.0.
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