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A marker was assessed as universal if its primers successfully amplified loci in all 16 species.
Root elongation from the swelling marker was assessed visually.
Performance of each marker was assessed by AU-ROC curve.
Caspase-mediated cytokeratin cleavage, an early apoptosis marker, was assessed by M30 antibody staining.
The prognostic strength of each marker was assessed using Cox proportional hazard regression model.
The validity of umbilical NGAL level as a diagnostic marker was assessed using sensitivity and specificity.
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Differences in phenotype changes (percentage) between genotypes at a given marker were assessed by a t-test.
In addition, the expression of typical chondrogenic markers was assessed by real-time reverse-transcription polymerase chain reaction analysis.
Neutrophils isolated from 12 healthy blood donors were incubated with either PACAP or VIP, and the expression of neutrophil cell surface markers was assessed using flowcytometry.
The predictive ability of the markers was assessed through areas under the corresponding ROC curves, which were estimated by 95%% confidence intervals.
The informativeness of markers was assessed by calculating polymorphic information content (PIC; Botstein et al. [1980]) using PowerMarker V3.0 (Liu and Muse [2005]).
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