Sentence examples for marker was amplified from inspiring English sources

A DNA module which includes the tandem epitope-encoding sequence and a selectable marker was amplified by PCR with primers that carry homologous extensions to the last portion and downstream region of the targeted gene.

The Kluyveromyces lactis (Kl) URA3 marker was amplified from plasmid pIGS05 (Scalcinati et al. 2012).

For CgCDR1 and PUP1 overexpression, the SAT1 marker was amplified using the primers SAT1-NotI (5'-ataagaatGCGGCCGCgtcaaaactagagaataataaag-3') and SAT1-BamHI (5'-gcaaaGGATCCggaccacctttgattgtaaatagt-3') and inserted into the NotI-BamHI restriction sites of pBluescript II SK to yield pSF30.

To express GFP under the control of the CgCDR1 promoter, the SAT1 marker was amplified using the primers SAT1-StuI (5'-ataagaatAGGCCTgtcaaaactagagaataataaag-3') and SAT1-BamHI (see above) and inserted into the StuI-BglII restriction sites of pBS-3xGFP TRP1 pBS-3xGFP TRP1g three tandemly fused GFP genes (3xGFP) to yield pSF104.

Each marker was amplified individually.

Each microsatellite marker was amplified singly.

A 3HA peptide coding sequence and URA3 selection marker were amplified from a pRS416-based plasmid (kind gift from Dr. Xuetong Shen).

In vervet monkeys, the DYS389 marker is amplified whether the individual is male or female.

A Chelex method was used for DNA extraction [ 58] and two mitochondrial (cytb and COI) and one nuclear (ITS2) marker were amplified.

From this vector, the salicylate-inducible NagR/pNagAa promoter, tac ribosomal binding site and Cm marker were amplified using primers RV165 and Cm_R_MluI (Table  2) and digested with Kpn2I and XmajI.

Indeed, none of these markers was amplified in the progeny isolates that, according to the graphical genotyping, lacked these particular LGs (Figure S3B and S3C).