Exact(3)
The rbcLa marker was aligned in Sequencher 4.8.
One marker was aligned with inappropriate chromosome (marker3869) and 112 markers (8%) revealed significant order errors.
However, only one marker was aligned to inappropriate chromosome and 112 markers revealed significant order errors, proving the practicability of the approach.
Similar(57)
Sequences for each marker were aligned in Muscle [30] using the default parameters.
Sequences for each marker were aligned using the program MUSCLE [ 41].
The high-quality consensus sequences obtained for each InDel marker were aligned and compared among accessions used.
In order to identify any amino acid substitution that may have functional implications between different virus haplotypes, the sequences from each marker were aligned to the corresponding gene sequence downloaded from NCBI GenBank, then translated in the corresponding frame using the standard genetic code.
To integrate this genetic map with the BAC-based physical map, the sequences of genetic markers were aligned with BES from the physical map using BLAST.
The high-quality consensus sequences thus obtained for each markers were aligned and compared among genotypes.
The markers were aligned in 12 linkage groups, with LOD scores ≥ 3.0.
SNP markers are aligned in the order defined by the cowpea consensus genetic map.
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