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This system presented a promising platform for the analysis of tumor genetic marker utilizing the built-in nucleic acid amplification module.
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Simple sequence repeat (SSR) markers, also known as microsatellite markers, utilize the variation for tandem repeats such as (CA)n repeats observed between genotypes [ 7].
ILP markers utilize the variation in the intron sequences and are the most easily recognizable type as it could be detected by PCR with primers designed on exons flanking the target intron.
Once conflicts among individual maps are detected, the correction in GTA is based on deleting some of the markers, whereas our approach is based on reordering the markers and utilizing the option of marker deletion only if the cost of consensus is high.
The ST111 binary vector also contained a selectable marker cassette utilizing the phosphinothricin acetyltransferase (BAR) gene under control of the nopaline synthase (NOS) promoter and terminator sequences.
Ebert and Peakall (2009) provide an excellent technical review on developing markers utilizing these resources.
Considering the importance of foxtail millet, functional significance of TEs and the necessity of TE-based markers in genotyping applications, this study was performed to identify the different classes of TEs and develop molecular markers by utilizing the sequence information of TEs.
Linkage analysis between the development of macroscopically evident arthritis and the homozygosity of chromosome-specific microsatellite markers by utilizing the N2 generation of back-crossing the F1 generation of SKG and Mus musculus castaneus to SKG mapped the skg locus to the centromeric portion of chromosome 1, with the lod score of the locus as infinite.
The other marker utilized was through anti-NeuN antibodies that bind specifically to the DNA-binding protein NeuN (Figure 2B).
To obtain a variant-based representation of the data, we repeated the network generation on marker scale by utilizing the set of variants associated with more than one distinct trait.
This vector contains the loxP sites, the thymidine kinase negative-selection marker utilized for random vector integration events, and FRT sites used to delete the neomycin resistance cassette.
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