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Dissection, single cell suspension, and analysis of tumors with minimum contamination by surrounding cells using multi-color flow cytometry allowed determining positive and negative staining for every marker using the fluorescence minus one (FMO) method (Supplementary Figure 1A).
We developed the SPL14-04SNP marker using the ST12-specific SNP to select the OsSPL14-ST12 allele.
Furthermore, we designed an indel marker using the 27-bp deletion sequence to genotype 153 RDP1 accessions (78 tolerant and 75 sensitive cultivars).
The AaargB gene was replaced with a selection marker using the AaargB deletion cassette 1 in pyrG13 and MR12 to elucidate the gene targeting frequencies.
Next, we developed the Gn1a-17 SNP marker using the tetra-primer PCR method (Additional file 2: Figure S2A) to discriminate G/A SNP in the promoter region (Fig. 1a).
Strikingly, we found no cases where both ER and GR of an expression trait mapped to the same marker, using the genome-wide significance cutoffs described above.
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Based on the genetic marker used, the monophyly of G. barreimiae cannot be confirmed.
Analysis of X-linked markers using the MLOD method from ASPEX also suggests linkage to the telomeric marker DXS6807.
40648_2018_105_MOESM1_ESM.mp4 Additional file 1. Whole-body motion to reach with both hands two AR markers using the proposed method.
Additionally, Wang et al (2011) developed gene-tagged markers using the difference in repeat number of di- or tri-nucleotides within GS3 for marker-assisted selection (MAS).
For example, Figures 3 and 9 show some results for user-selected markers using the image-based weight assignment from [36].
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